Time: 12:20 - 12:40
Fluorescence Recovery after Photobleaching (FRAP) and Fluorescence Loss in Photobleaching (FLIP) are microscopy methods for visualization of molecular transport processes in living cells. While in FRAP experiments the local recovery after photobleaching is investigated, a FLIP sequence observes fluorescence loss after repeated bleaching over time and throughout the entire cell.
In this contribution we simulate both FLIP and FRAP experiments on segmented cell images based on the reaction-diffusion system from [1,2]. The PDE model is solved by a Discontinous--Galerkin method implemented in FEniCS [3].
The simulation results are compared to in vivo FLIP and FRAP microscopy images using the Enhanced Green Fluorescent Protein (eGFP). Also simulated FRAP recovery curves will be compared to experimental data.
[1] Hansen, Schroll and Wüstner: Computational Modeling of Fluorescence Loss in Photobleaching, Comp. Vis. Sci. 17, 2015, pp 151--166.
[2] Hansen, Schroll and Wüstner: A Discontinuous Galerkin Model for Fluorescence Loss in Photobleaching, preprint IMADA, SDU, 2016. (submitted)
[3] Logg, Mardal, Wells, et.al.: Automated Solution of Differential Equations by the Finite Element Method, Springer, Heidelberg, 2012.
