Obtaining functionally intact primary human liver sinusoidal endothelial cells (hLSECs)

Bård Smedsrød

UiT-The Arctic University of Norway

Professor and head of Vascular Biology Research Group, University of Tromsø, Norway. Has been active in the field of liver sinusoidal cells, in particular the liver sinusoidal endothelial cell (LSEC), since 1981. Developed method of high yield isolation and cultivation of LSECs from rat in 1981. Discovered the central physiological scavenger function of LSECs, the cell type that is today known to be the major site of uptake of blood borne physiological macromolecular ligands for the mannose receptor, stabilins, and the Fc gamma receptor IIb2. Revealed the presence of the vertebrate scavenger cell, which is present as LSECs in land based vertebrates,  endocardium and kidney sinusoids in bony fish, and gill capillary endothelial cells in phylogenetically younger vertebrates. Uncovered the concept of the dual cell principle of waste clearance, i.e. preferential uptake from the circulation of soluble macromolecules and nano particles by clathrin mediated endocytosis in LSECs, and of larger particles mediated by phagocytosis in Kupffer cells.     


Protocols published for isolation and cultivation of hLSECs encompass long-term cultivation, and often trypsinization and passaging. Moreover, experiments are generally carried out with hLSECs that have been maintained for... [ view full abstract ]


  1. Cristina Ionica Øie (UiT-The Arctic University of Norway)
  2. Aernout Luttun (KU Leuven)
  3. Willeke de Haan (KU Leuven)
  4. Åsmund Fretland (Oslo University Hospital)
  5. Bård Ingvald Røsok (Oslo University Hospital)
  6. Inigo Martinez (UiT-The Arctic University of Norway)
  7. Bård Smedsrød (UiT-The Arctic University of Norway)

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OS3 » Session 3 LSEC (11:15 - Thursday, 15th June, Aula Maxima, Ground Floor)