Surface-enhanced Raman spectroscopy (SERS) for environmental microorganism analysis: antibiotic resistance, nanotoxicity, and biofilm
Li Cui
Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences
Dr. Li Cui is an Associate Professor in Institute of Urban Environment. She got her Bsc and PhD degree from Chemistry department of Xiamen University, China. Her research interest includes application of Raman, surface-enhanced Raman spectroscopy and stable isotope labelling techniques in microorganism-related studies, including antibiotic resistance, nitrogen cycle, biofilm, nanotoxicity. She has published more than 20 papers in journals of Analytical Chemistry, Water Research, Journal of the American Chemical Society, Current Opinion in Biotechnology etc, and has served as reviewers of many journals including Analytical Chemistry, Environmental Science and Technology, Chemical Communications etc.
Abstract
Surface-enhanced Raman spectroscopy (SERS) is a non-destructive moleculal-vibration spectroscopy capable of providing whole-organism fingerprinting information of microorganism and their sensitive phenotypic responses to... [ view full abstract ]
Surface-enhanced Raman spectroscopy (SERS) is a non-destructive moleculal-vibration spectroscopy capable of providing whole-organism fingerprinting information of microorganism and their sensitive phenotypic responses to various external factors. Moreover, the strong electromagnetic enhancement provided by Ag or Au nanoparticles endows SERS extra advantages over normal Raman spectroscopy in ultrahigh sensitivity down to single-molecules level, rapid detection, surface information characterization, and fluorescence-quenching ability. Here, the applicability of SERS in studying heavy metal arsenic (V)-enhanced antibiotic resistance, nanotoxicity of Ag and ZnO NPs to bacteria, as well as variation of bacterial species and chemical compositions of biofilm during the development will be presented.
Bacterial antibiotic resistance poses a threat to global public health. Independent and robust SERS spectral changes representing phenotypic bacterial responses, combined with multivariate analysis, clearly identified that As(V) enhanced antibiotic resistance to tetracycline (Tet).1 For nanotoxicity studies, SERS in-situ revealed size-, media-, time-, and does-dependent toxic responses of bacteria to Ag and ZnO nanoparticles.2 For biofilm studies, SERS indicated a dynamic change of dominant bacterial species within the biofilm with culture time, based on the distinguishable SERS features of two bacteria.3 SERS also achieved layer-by-layer interrogation of the chemical composition of biofilm on nanofiltration membrane (biofouling) during both development and removal processes and revealed biofilm composition-dependent cleaning efficiency.4
(1) Cui, L. et al., Anal. Chem. 2016, DOI: 10.1021/acs.analchem.5b04490.
(2) Cui, L. et al., Anal. Chem. 2013, 85, 5436-5443.
(3) Chen, P. Y. et al., J. Membr. Sci. 2015, 473, 36–44.
(4) Cui, L. et al., Water Res. 2015, 87, 282-291.
Authors
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Li Cui
(Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences)
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Yingjiao Zhang
(Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences)
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Kaisong Zhang
(Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences)
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Yong-Guan Zhu
(Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences)
Topic Area
Please tick the most appropriate topic for your submission: Sensors and Technologies
Session
OS-6D » Microbiology (15:30 - Tuesday, 16th August, Mc Munn Theatre)
