The roles of OsNIPs gene's transcription regulation in arsenic absorption and accumulation in rice (Oryza Sativa L.)

In order to study the transcription regulation of arsenic uptake and accumulation of rice (Oryza sativa L.), in different phosphate concentrations, the rice seedlings are treated with arsenate (As (V)) and arsenite (As (III))... [ view full abstract ]

In order to study the transcription regulation of arsenic uptake and accumulation of rice (Oryza sativa L.), in different phosphate concentrations, the rice seedlings are treated with arsenate (As (V)) and arsenite (As (III)) (0 µM, 10µ M, 100 µM). We determine the change of rice biomass, arsenic accumulation, AsA-GSH redox system activity and gene expression of OsNIPs family, also determine the short-term absorption kinetics of As (V) andAs (III) of rice.The results showed that phosphorus deficiency (-P) can promote root biomass under As (III) stress but inhibited root biomass under As (V) stress. The high concentration of As (III) (100 µM) stress can promote the expression of the OsNips family, except for OsNip2;2. OsNip1;2, OsNip1;3, OsNip1;4, and OsNip3;3 may participate in the absorption of As (III) in root, phosphorus deficiency can improve the absorption rate of arsenic in rice. OsNip1;2, OsNip2;1, OsNip2;2, OsNip3;1, and OsNip3;2 may participate in root-to-shoot arsenic translocation. -P could improve root-to-shoot arsenic transport and increase the accumulation of As (III) in plants. -P also was observed enhance the activity of AsA-GSH redox system to reduce the lipid peroxidation degree.
Key Words: Arsenate, Arsenite, rice, OsNips, transcription