The Functions of OsPHT7 gene involved in arsenic absorption and accumulation in Oryza sativa L

In order to fully understand the function of OsPHT7 (high affinity phosphate transporter gene in Oryza sativa L.) in phosphorus and arsenic absorption and accumulation, the effect of OsPHT7 gene expresssion on arsenic and... [ view full abstract ]

In order to fully understand the function of OsPHT7 (high affinity phosphate transporter gene in Oryza sativa L.) in phosphorus and arsenic absorption and accumulation, the effect of OsPHT7 gene expresssion on arsenic and phosphorus absorption was investigated in this study.
OsPHT7 was cloned from Indica rice. Then, genes were connected with a shuttle vector pPIC9K and express successfully in Pichia pastoris GS115. The rice seedlings and yeast were treated with different arsenate As(Ⅴ) and arsenite As(III) treatments with or without phosphate. The result showed OsPHT7 play an important role in the As(Ⅴ) absorption and accumulation of both in rice and yeast. The absorption of As (Ⅴ) and P were the cooperative relationship. According to OsPHT7 sequence, one of predicted arsenic binding sites of the transporter was site-directed mutagenesis from glutamine into proline. The experiment also proved that glutamine could be a key amino to bind As(Ⅴ).
Key Words：arsenic, phosphate transporter gene, Oryza sativa, Pichia pastoris