Effectively Anticancer Drug Concentration Gradients by Using 3D Microfluidic Chip

A 3D microfluidic chip for resistance of H460 lung cancer cells (LCC) was demonstrated. H460 LCC could be restrained by the injection of different curcumin concentration and investigated in different microenvironmental pH... [ view full abstract ]

A 3D microfluidic chip for resistance of H460 lung cancer cells (LCC) was demonstrated. H460 LCC could be restrained by the injection of different curcumin concentration and investigated in different microenvironmental pH conditions. In the range of curcumin concentration of 57.2-100 μg/mL, the average apoptosis rate (AR) of H460 LCC were 53.6%, 58.4%, and 98.0% for pH=6.5, 7.4 ,and 8.5 microenvironments, respectively. The resistant efficiency of H460 LCC could be enhanced substantially at pH=8.5 microenvironmental condition. These results pave a way for using 3D microfluidic chip in cell biology application.