Development of material-binding proteins fused device to improve sensitivity of biosensor

Biosensor is used early diagnosis in medical setting. Many biosensors use antibodies for recognizing marker proteins and the antibodies should be immobilized on sensor chips that transduce capturing action to optical or... [ view full abstract ]

Biosensor is used early diagnosis in medical setting. Many biosensors use antibodies for recognizing marker proteins and the antibodies should be immobilized on sensor chips that transduce capturing action to optical or electrical signals. High density and homogeneous orientation of immobilized antibody increases sensitivity for biosensor. In general, antibodies are chemically immobilized on sensor chips; however, the chemical method can’t control the orientation of antibody on the chips and disrupt there’s construction.

Recently, we devised a single variable domain of the heavy chain camel antibody (VHH) with high affinity for inorganic material surface. Use of the VHH for an interface between sensor chip and recognition molecule is expected for highly sensitive detection, because it can immobilize antibody with high density on the surface of material chip and it also needs no chemical modification.

 In this study, we constructed a fusion protein by fusing various antibodies to C-terminal of material -binding VHH and tried to make the wide use biosensor which can detect various maker proteins sensitivily. Consequently, sensitivity of target detection increases by using fusion protein compared with physical adsorption.