Immobilization of enzyme on porous silicon for electrochemical detection of organophosphorous compounds

In this work, porous silicon (PSi) surface was functionalized by a multistep process in order to immobilize a specific enzyme, acetylcholinesterase (AChE). AChE hydrolyses acetylthiocholin (ATCh) or substrate in thiocoline and... [ view full abstract ]

In this work, porous silicon (PSi) surface was functionalized by a multistep process in order to immobilize a specific enzyme, acetylcholinesterase (AChE). AChE hydrolyses acetylthiocholin (ATCh) or substrate in thiocoline and acetic acid. The modified AChE electrode is used for the electrochemical detection of organophosphorous compounds. The electrochemical detection using the modified electrode was perfomerd in presence of Malathion at different concentrations using Acetythiocholine Iodide (ATCl Iodide) as enzyme substrate. A decrease of oxidation peak current was observed, by increasing the Malathion concentrations, and this was due to the blocking of active sites of the AChE.