Peroxidase-like Activity of Apoferritin Paired Copper(II) Nanoparticles and Its Biomemory Applications

Bionanomaterials are increasingly attracted in recent years due to the unique behaviours in biosensors, bioassays, nanoelectronics, drug delivery system and biomedical imaging. Ferritin, a nature bionanomaterial, is an iron... [ view full abstract ]

Bionanomaterials are increasingly attracted in recent years due to the unique behaviours in biosensors, bioassays, nanoelectronics, drug delivery system and biomedical imaging. Ferritin, a nature bionanomaterial, is an iron storage and detoxification globular protein, composed of 24 homologous polypeptide subunits of the heavy chain (H-ferritin) and light chain (L-ferritin).
Bionanomaterials have been prepared using apoferritin biomolecules embedding of metal atoms into lattice of this protein. These bionanostructures have obtained using photosensitive amino acid-monomers linkage method (ANADOLUCA). In this study, the synthesis of nanoapoferritins has achieved in the presence of bis (2-2’-bipyridil) MATyr-MATyr-rutenyum (II) photosensitive monomers by microemulsion. Then, Cu(II) metal has organised into lattices of apoferritin nanostructures and these multi functional structures have characterised by transmission electron microscopy (TEM).
Michaelis-Menten kinetics of bionanomaterials have improved using 3.3′,5.5′-tetramethylbenzidine (TMB) which is the substrate of HRP. The effect of pH, temperature and subsrate concentrations on the activity of these bionanomaterials have analyzed. The memory function of the fabricated biodevice has validated using cyclic voltammetry (CV) and chronoamperometry (CA).

Key word: Apoferritin, Biomemory, Bionanostructure, Peroxidase Activity