Large-scale silver-modified nanofluorapatite – bactericidal/cytotoxicity evaluation and physical characterization

In this report the physical and biological properties of new biomaterial: silver-doped fluorapatite were investigated. The aim of the study was to evaluate its bactericidal effect and the cytotoxicity on eukaryotic cells.... [ view full abstract ]

In this report the physical and biological properties of new biomaterial: silver-doped fluorapatite were investigated. The aim of the study was to evaluate its bactericidal effect and the cytotoxicity on eukaryotic cells. Slightly modified method for the synthesis of apatite, doped with silver ions (for improving bactericidal efficacy) gives rise to the production of new promising biocomponent. The applications of fluor- and hydroxyapatites doped with silver occurred to be toxic to Staphylococcus aureus and have strongly indicated its antimicrobial activity. Importantly, material based on fluorapatite demonstrated highest biocidal effect in comparison to widely known hydroxyapatite. The structure of materials were studied by FTIR, Raman spectroscopy, TEM and Zeta potential analyses. The perspective of the future would be a using of such biomaterial as bone filler in medical industry e.g. implants or in dentistry with its bactericidal activity. Regarding to their use in medicine, this materials have not to be toxic to healthy cells. Thus, it have been also assessed the degree of cytotoxicity of modified apatites to eukaryotic cells.