SYNTHESIS AND CHARACTERIZATION OF PEPTIDE LOADED NANOPARTICLES FOR USE IN NEW GENERATION VACCINE SYSTEMS

Introduction: Recent studies with polymeric carriers have gained importance as they provide advantages to synthetic peptides that are frequently used in vaccine technology such as higher immunity, longer survival in biological... [ view full abstract ]

Introduction: Recent studies with polymeric carriers have gained importance as they provide advantages to synthetic peptides that are frequently used in vaccine technology such as higher immunity, longer survival in biological system and more stability against destructive factors. Among these developed carriers, nanoparticles are extremely popular in recent years, especially with their cellular dimensions, controlled release possibilities, high biocompatibilities.

Methods: In our studies, antigenic peptide epitopes of a current disease are encapsulated to albumin, PLGA or PLA etc. nanoparticles. Synthesized nanoparticles were characterized by methods of ZetaSizer, scanning electron microscopy (SEM) and Fourier transform infrared (FTIR) spectroscopy. Loading and capsulations yields were calculated, bio-release experimentations were performed.

Results: As a result of the obtained data, it was seen that highly efficient, biodegradable and biocompatible nanostructured vaccine prototypes have been successfully prepared.

Discussion: Within the scope of our studies, it is expected that the systems in which synthetic peptides with antigenic properties are encapsulated in nanoparticles will increase the biocompatibility of the peptides and increase the immunogenic properties in the result of controlled release and decrease the side effects caused.

Keywords: Nanoparticles, synthetic peptides, vaccine prototypes

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