UV-spectroscopy Method for Detecting the Chitosan Nanoparticles Formation

This research work reviews the UV spectroscopic technique developed for the detection of formation low molecular weight (LMW) chitosan mucoadhesive nanoparticles loaded by recombinant human keratinocyte growth factor... [ view full abstract ]

This research work reviews the UV spectroscopic technique developed for the detection of formation low molecular weight (LMW) chitosan mucoadhesive nanoparticles loaded by recombinant human keratinocyte growth factor (rHuKGF). These nanoparticles were prepared by the ionic gelation method. This method is one of the most widely accepted protocols for chitosan nanoparticles synthesis and it produces colourless and cloudy polydisperse chitosan nanoparticles.  In the research work, the rHuKGF in tripolyphosphate (TPP) solution was added in drops to produce rHuKGF loaded LMW chitosan nanoparticles.  The ionic gelating agent TPP will react with LWM chitosan at above pH5.2 and form the nanoparticles.  During the formation of LWM chitosan nanoparticles, the rHuKGF will be entrapped in the nanoparticles. The main issue is that of maximizing the loading capacity of rHuKGF in LWM chitosan nanoparticles and avoiding its wastage. It depends on how much amount of rHuKGF in TPP solution was added to convert all the chitosan molecules in the solution to form as nanoparticles.  A possible method for detecting the formation of these rHuKGF loaded chitosan nanoparticles is done by observing their light scattering property. In this study formation of nanoparticles was accurately detected by using UV-spectrophotometer at 231 nm and avoided the excessive addition of rHuKGF.

ACKNOWLEDGMENT:
This project (Ref: FRGS/2/2014/SKK02/UCSI/02/1) is supported by Department of Higher Education, Ministry of Education Malaysia, under the Fundamental Research Grant Scheme (FRGS) Malaysia.