Eradication of Glioblastoma Multiforme stem-cells with Immunotargeted-molecular-robotic-NANOSURGERY (ITMRN)
Abstract
Introduction:GBM stem cells are resistant to conventional treatment forming recurrent-tumors via angiogenesis .Thus,they are considered primary targets for novel therapeutic strategies for GBM. Methods:We obtain relapsed... [ view full abstract ]
Introduction:GBM stem cells are resistant to conventional treatment forming recurrent-tumors via angiogenesis .Thus,they are considered primary targets for novel therapeutic strategies for GBM.
Methods:We obtain relapsed chemo-radioresistant GBM stem cells overexpressing CD133 from which we develop a GBM model that we treat with immunotargeted pegylated silver nanobots(PEG-AgNPs) which are covalently conjugated to anti-CD133 MAbs for targeting Brain-Cancer-Stem-Cells(BCSCs) after receptor-mediated-endocytosis.Then,we treat them with radiofrequency-dielectric-heating(RFDH) as a novel postsurgical-adjuvant-treatment.
Results:Post-treatment,the silver nanobots AgNPs release Ag+ ions which disrupt mitochondrial-respiratory-chain(MRC) blocking ATP-synthesis by production of ROS which damage the DNA of CSCs leading to cell-cycle arrest at G2/M phase and type-1 PCD of stage-D2 or apoptosis.The capacitive nonionizing radiofrequency-dielectric-heating of 13.56MHz exerts hyperthermic cytotoxicity increasing the intracellular-Temperature of BCSCs up to 50 degrees Celcius leading to the necrosis of Brain-Cancer-Stem-Cells(BCSCs).
Conclusion:This immunotargeted molecular robotic nanosurgical approach may be used as an adjuvant treatment against GBM which is resistant to conventional chemo/radiation therapy by eradicating its cancer-stem-cells.
Authors
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Prof John Giannios
(Dept of Translational Cancer Medicine,Int Assoc of Personalised Perioperative Medicine and Nanosurgery, Hellenic and International Society of Molecular and Genomic Medicine, Ath,GR prdrjng@gmail.com)
Topic Area
Adult Gliomas
Session
OS-22A » Parallel Session A: Science (09:40 - Thursday, 22nd June, Pentland East)
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