Probing glioblastoma infiltration into healthy tissue by magnetic resonance perfusion imaging: a quantitative MRI evaluation
Abstract
The ability of tumour cells to invade healthy brain tissue is a major obstacle to successful treatment of Glioblastoma (GBM). This makes complete removal of the tumour by surgery impossible, leading to high recurrence rates,... [ view full abstract ]
The ability of tumour cells to invade healthy brain tissue is a major obstacle to successful treatment of Glioblastoma (GBM). This makes complete removal of the tumour by surgery impossible, leading to high recurrence rates, and reduces the accuracy of target volume delineation for radiotherapy planning. Providing with a wide range of imaging modalities, Magnetic Resonance Imaging (MRI) is an important tool for GBM diagnosis and characterisation. However, conventional MRI techniques fail to detect regions of low tumour cell density that may be responsible for subsequent tumour recurrence, while novel techniques are often not reliable due to a lack of robust evaluation protocols.
Invading tumour cells often progress along blood vessels and recent results indicate that even individual cells can disrupt the normal function of the blood brain barrier (BBB), providing an opportunity to detect tumour invasion at its earliest stages. Our research focuses on the development of perfusion imaging techniques, and their quantitative assessment in detecting low tumour infiltration regions on mouse GBM models presenting highly invasive tumour margins. A high SNR multiple adiabatic boli Arterial Spin Labelling technique (mbASL) was implemented and optimised for rodent brain perfusion imaging and its ability to probe tumour invasion was evaluated by comparison with standard MRI techniques (T2 and diffusion weighted imaging) and immunohistochemistry sections. To achieve a quantitative MRI evaluation, multiple histological slices (HLA stain for human GBM) were cut in the MRI plane, registered and stacked to account for MRI slice thickness. This approach, leading to the production tumour density maps in the MRI plane, allows going beyond the standard evaluation tests to consider a voxel-to-voxel comparison between MRI and histology. Our results confirm the limitations of standard imaging modalities in probing low tumour densities and demonstrate the existence of a quantitative relation between glioblastoma invasion and brain perfusion.
Authors
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Antoine Vallatos
(Glasgow experimental MRI centre, Institute of Neuroscience and Psychology, University of Glasgow)
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Joanna Birch
(Institute of Cancer Sciences, University of Glasgow)
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Haitham Al-Mubarak
(Glasgow experimental MRI centre, Institute of Neuroscience and Psychology, University of Glasgow)
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Lindsay Gallagher
(Glasgow experimental MRI centre, Institute of Neuroscience and Psychology, University of Glasgow)
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Lesley Gilmour
(Institute of Cancer Sciences, University of Glasgow)
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William Holmes
(Glasgow experimental MRI centre, Institute of Neuroscience and Psychology, University of Glasgow)
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Anthony Chalmers
(Institute of Cancer Sciences, University of Glasgow)
Topic Area
Imaging
Session
OS-22F » Parallel Session F: Clinical (16:00 - Thursday, 22nd June, Prestonfield)
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