In vitro activation and maturation of primordial follicles of trans men is currently limited by inadequate support of the preantral follicle growth

Chloë De Roo

Division of Reproductive Medicine, Department of Gynaecology, Ghent University Hospital, 9000 Ghent

Chloë De Roo concluded her medical studies at the University of Ghent in 2012. She is combining her specialization in gynaecology – obstetrics with fundamental and applied scientific research at the Centre for Reproductive Medicine at the Ghent University Hospital in order to obtain a PhD degree in fertility preservation.

Background

Ovarian tissue cryopreservation is an interesting fertility preservation option for trans men as it does not require controlled ovarian stimulation, involving female hormone rises and vaginal ultrasound monitoring. Future use... [ view full abstract ]

Aim(s)

In order to follow the primordial follicle activation and maturation during in vitro culture of frozen thawed ovarian tissue of trans men, detailed histological follicle observations were performed during the in vitro culture. [ view full abstract ]

Main Outcome Measures

Cryopreserved ovarian tissue, donated for research, of 3 trans men (23.3 +/- 1.53 years old) was thawed and prepared for in vitro culture. All of them underwent a hysterectomy with bilateral oophorectomy after a period of... [ view full abstract ]

Methods

The paraffin embedded ovarian cortical tissue piece was serially sectioned at 5µm, resulting in 10 slices, and stained with (Mayer) hematoxylin (Merck, Overijse, Belgium) and eosin (Thermo Scientific, Erembodegem, Belgium).... [ view full abstract ]

Results

The mean count of the 2 observers showed a total of 2099.00 follicles, with a mean of 699.67 + 242.06 follicles per patient (N=3) and a mean of 524.75 +/- 166.04 follicles per culture day (N=4). The total number of follicles... [ view full abstract ]

Conclusion

The current culture model, as described by E. Telfer (2008), nicely supports the primordial follicle activation in in vitro culture of frozen thawed ovarian tissue of trans men. However, a clinically useful culture system... [ view full abstract ]

Authors

Chloë De Roo (Division of Reproductive Medicine, Department of Gynaecology, Ghent University Hospital, 9000 Ghent), Kelly Tilleman (Division of Reproductive Medicine, Department of Gynaecology, Ghent University Hospital, 9000 Ghent), Sylvie Lierman (Division of Reproductive Medicine, Department of Gynaecology, Ghent University Hospital, 9000 Ghent), Guy T'Sjoen (Ghent University), Ria Cornelissen (Department of Basic Medical Science, Faculty of Medicine and Health Science, Ghent University, 9000 Ghent), Steven Weyers (Department of Gynaecology, Ghent University Hospital, 9000 Ghent), Petra De Sutter (Division of Reproductive Medicine, Department of Gynaecology, Ghent University Hospital, 9000 Ghent)

Topic Area

Oral & Poster Topics: Endocrinology

Session

OS-2C » Endocrinology II (11:00 - Friday, 7th April, Atlantic 3)

Presentation Files

The presenter has requested not to share their files.