Study Effects of Synchronize Utilize of Gamma-ray and Silver Nano Particles Treatment on Human Prostate Cancer Cells

Background: Prostate cancer is the second most common cancer in America which causing great harm and waste cost. Furthermore more prostate cancer treatment is ineffective. Purpose of this study was to evaluate the effects of... [ view full abstract ]

Background: Prostate cancer is the second most common cancer in America which causing great harm and waste cost. Furthermore more prostate cancer treatment is ineffective. Purpose of this study was to evaluate the effects of therapy and the rate of increase of the absorbed dose of gamma radiation with silver nano particles in treatment of prostate cancer.
Materials & Methods: DU145 cell line originating from Human prostate cancer was purchased from Pasteur Institute. After thawing of defreezed samples, cells were incubated with DMEM medium and 15% FBS serum was added. For preventing contamination amphotericin B in 25 μM/ml was added. Cells were incubated over a period of 3 to 5 days to reach a good Con-fluency then cells divided into 4 groups. Tentatively assigned to the control group, the second experimental group treated with gamma irradiation at a doses of 2, 6 and 10 Gray(Gy), the third group treated with 53 μ g / ml silver nano particles, The fourth group includes simultaneous treatment with gamma doses and silver nano particle. Cell groups studied by staining with trypan blue as well as by MTT assay (ELISA) reader.
Results: The results showed the use of gamma rays and silver nano particles caused a significant reduction in the number of cancer cells in the treated groups compared to the other treatment groups and a control group. Using of silver nano particles as a radio sensitize and radiation therapy in prostate cancer cell lines DU145 resulted in the increase of the gamma-ray photon energies of 6 and 10 Gy
Discussion: The gamma photon radiation to the tumor cells were incubated nano particle probability Photoelectric process as a process when handling the extremely high energy gamma photons hitting the silver nano particles to cancer cells, photo electron and Auger electrons (Auger Electrons) and secondary electrons produced by secondary particles can be It will not be very effective, leading to failures in the field of DNA damage and cell death.
Keywords: Prostate cancer, gamma - ray , Silver nano particles, DU145, Cell Culture