A novel immunosensor based on polypyrrole electroless deposition on silicon nitride substrates: Interleukine-10 detection

We report in present study about development of a novel strategy of anti-human interleukin-10 (anti-IL-10) antibody immobilization onto polypyrrole (PPy) modified capacitance substrate. Here, we propose the development of a... [ view full abstract ]

We report in present study about development of a novel strategy of anti-human interleukin-10 (anti-IL-10) antibody immobilization onto polypyrrole (PPy) modified capacitance substrate. Here, we propose the development of a biosensor based on silicon nitride (Si3N4) chemically modified by a deposition of a PPy layer. In order to ensure adhesion of the conductive organic polymer to the substrate, a silane-pyrole reagent (SPy) N-(3-trimethoxysilylpropyl) pyrrole was used to create a covalent bridge between the silicon substrate and the layer of PPy (Scheme 1). The surface morphology of the composite Si3N4-P(SPy-Py) was studied by scanning electron microscopy (SEM) and infrared spectroscopy (FTIR). The electrochemical behavior of the novel composite Si3N4-P(SPy-Py) was characterized by cyclic voltammetry (CV) and electrical impedance spectroscopy (EIS). The (Si3N4)-P(SPy-Py) composite was subsequently electrochemically modified by diazonium salts (4-Aminophenylacetic acid) (CMA) for the immobilization of specific antibodies (anti-human interleukin-10 monoclonal antibody) by carbodiimide chemistry. The configured immunosensor successfully detected human IL-10 in the range of 1-50 pg/mL. This new composite shows a high sensitivity and a novel approach for the development of new strategies for biosensor development and detection.