Effect of Nanosilver with salicylic acid & nano salicylic acid pre-treatment on Antioxidant responses in Dracocephalum moldavica L

Nanoparticles (NPs) with different composition, size, and concentration have been reported to influence growth and development of various plant species with both positive and negative effects. Ag-nanoparticle is important for... [ view full abstract ]

Nanoparticles (NPs) with different composition, size, and concentration have been reported to influence growth and development of various plant species with both positive and negative effects. Ag-nanoparticle is important for its characteristic physiochemical and biological properties. Dracocephalum moldavica L. is well known as medicinal plants because of its biological and pharmacological properties. Salicylic acid (SA) is an important signal molecule modulating plant response to stress. In here, we investigated some antioxidative responses of D. moldavica under Ag-NPs pretreated with different SA and nano-SA concentrations. The study was carried out in a randomized block design with 3 replications in four levels of SA and nano SA (0, 20, 60, 80 mM) and six Ag-NPs treatments (0, 10 ,20, 40, 60, 80 PPm) for 21 days. AgNPs significantly induced oxidative stress marker such as H2O2 content in a concentration dependent manner. The phytotoxicity of AgNPs led to an increase in catalase and peroxidase activities and synthesis of antioxidant compounds such as carotenoids, proline and total soluble carbohydrate. Further results showed that SA and nano-SA pretreatments ameliorates the Ag- phytotoxicity, which nano-SA was more effective than SA in this manner.