In vivo anti-inflammatory activity by trans-resveratrol loaded-solid lipid nanoparticle for skin disorders

Trans-resveratrol (RES) presents important action to prevent and treat skin disorders. Cutaneous administration of RES has being explored in order to find it on their site of action [1]. Solid lipid nanoparticles (SLN) due to... [ view full abstract ]

Trans-resveratrol (RES) presents important action to prevent and treat skin disorders. Cutaneous administration of RES has being explored in order to find it on their site of action [1]. Solid lipid nanoparticles (SLN) due to interaction characteristics with skin have being used to drug delivery system for topical application [2]. The aim was verify in vivo anti-inflammatory activity of SLN with RES for cutaneous application therapy. The formulations developed were composed by stearic acid (SA) as solid lipid, polysorbate 80 (P80) and soy phosphatidylcholine (SP) as surfactants, and poloxamer 407 (P407) and glycerin as stabilizers (F1). Cetrimonium bromide (CB) was added as cationic surfactant to promote positive superficial charge (F2). The formulations was add of 0.25% RES (F1.RES and F2.RES). The anti-inflammatory and antinociceptive activities of SLNs were measured by carrageenan-induced paw edema and paw pressure test in mice, respectively [3,4]. This research was approved by Ethics Research Committee of UNESP (n° 68/2015). The average hydrodynamic diameters were 195.0 ± 3.34 nm, 241.3 ± 48.33 nm, 159.15 ± 4.78 nm e 158.25 ± 33.92 nm to F1, F1.RES, F2 e F2.RES, respectively. Zeta potentials (mV) were -25.5 ± 1.01; -26.0 ± 1.67; 30.6 ± 1.13 e 30.0 ± 1.85 mV for F1, F1.RES, F2 e F2.RES, respectively. Entrapment efficacy was analyzed using validated analytical methodology and both formulations (F1.RES and F2.RES) present ~50% of RES entrapped. RES solution (1:1 ethanol and water) and F2.RES presented reduction of nociception similar to dexametasone commercial cream, which suggest potent anti-inflammatory activity of RES and F2.RES. Stratum corneum have a negative surface charge that permits greater bioadhesion of positive particles surface, which could explain the increase of antinociceptive activity by F2.RES compared to F1.RES. The results demonstrated the importance to investigate the RES action when it is entrapped in drug delivery system for topical application.

References
[1] Ndiaye, Mary, et al. Archives of biochemistry and biophysics 508.2 (2011): 164-170.
[2] Wissing, Sylvia A., and Rainer H. Müller. International Journal of Pharmaceutics 254.1 (2003): 65-68.
[3] RANDALL, L. O. Arch Int Pharmacodyn, 111 (1957): 409-419.
[4] SANTOS-NOGUEIRA, E. et al. Journal of neurotrauma 29.5 (2012):898-904.