Carbon nanotubes (CNTs) have unique nanostructures and physicochemical properties. For example, when CNTs are irradiated by visible lights, near-infrared (NIR) photoluminescence is observed. On the other hand, when CNTs are... [ view full abstract ]
Carbon nanotubes (CNTs) have unique nanostructures and physicochemical properties. For example, when CNTs are irradiated by visible lights, near-infrared (NIR) photoluminescence is observed. On the other hand, when CNTs are irradiated by NIR lights, significant adsorption of NIR is observed. There is another unique phenomenon in fluorescent spectroscopy. When some fluorescent dyes adsorbed on CNT surfaces, fluorescence from the dyes are almost quenched even when the dyes are irradiated by suitable lights for excitation. Using the physicochemical properties, various new methods for nanobiosensing such as detecting single-mismatch of DNA sequences have been reported by various authors.
We have reported nanoscopic characterization of hybrids of DNA and CNTs (DNA-CNT hybrids) in order to obtain fundamental information of interaction between DNA and CNTs. [1-6] Furthermore, we have investigated reactions of DNA binding proteins with DNA-CNT hybrids to establish biochemical reactions on CNT surfaces.
In this paper, we systematically studied optical responses of DNA-CNT hybrids under various conditions. UV-vis, NIR, photoluminescence, and fluorescent spectroscopies were applied to the same samples for comparison. As a result, we found that DNA-CNT hybrids can detect changes of the spectra caused by minute perturbation such as injection of chemicals. Our results will be a basis of application of CNTs to nanobiosensing. In addition, causative proper use of the several optical measurements dependent on research purposes will be discussed.
[1] T Hayashida and K Umemura, Colloids Surfaces B, 143, 526-531(2016).
[2] K Umemura. Nanomaterials, 2015, 5(1), 321-350(2015).
[3] S Oura, M Ito, D Nii, Y Homma, K Umemura. Colloids Surfaces B, 126, 496–501(2015).
[4] D Nii, T Hayashida, Y Yamaguchi, S Ikawa, T Shibata, K Umemura, Colloids Surfaces B, 121, 325-330(2014).
[5] D Nii, T Hayashida, K Umemura. Colloids Surfaces B, 106, 234–239(2013).
[6] T Hayashida, K Umemura. Colloids Surfaces B, 101, 49-54(2013).
Biological & medical nanodevices and biosensors , Bionanocatalysis and nanobiosystems
