Introduction:Metal nanoparticles are an effective antibacterial solution as an efficient and safe solution for the standard additive (such as the aromatic compound) in food, pharmaceuticals, cosmetics, ...Nanoparticles have a... [ view full abstract ]
Introduction:
Metal nanoparticles are an effective antibacterial solution as an efficient and safe solution for the standard additive (such as the aromatic compound) in food, pharmaceuticals, cosmetics, ...
Nanoparticles have a single chemical-physical property for microbiological application (such as the antimicrobial agent for infectious diseases).
In this preliminary study fifteen different species of procariota microorganism are used for tested the efficacy of silver NPs. Eucariota microorganism selected rapresent a major severe compliance for one specific pathology (cistic fibrosis).
The synthesis process is total controlled by electrical equipment assembled and designed in our laboratory.
Methods:
Electrochemical synthesis was used for produced an silver nanoparticle solution with pH < 8 and was loaded with analytical techniques (TEM, SEM-XRD, UV-Vis, Light Dispersion) and tested with microbiological microorganisms (> 10 different species: pseudomonas, coli, streptococcus ...).
Microbiological data are important evidence for application to pathological diseases.
All synthesis proces are controlled by supervisor on electronic device assembled and designed in our laboratory; this controller is necessary for reproducible method.
Parameters contolled: temperature, reaction time, time stirrer and electrochemical parameter (such as Intensity-mA/cm, ddp, wave form).
Results and Discussion:
We present a fast, reproducible and inexpensive electrochemical method for synthesizing a smoler silver nanoparticle (8.34 ± 4.09 DS particles) with negative charge (-50 ± 5 mV) without chemical stabilization (without citrate, ascorbate ...) in ultra pure aqueous solution .
Low reaction time (< 10 minute) and silver concentration is 30-40 ppm depending of running time.
The solution is stable for 3 months if is capped under nitrogen and stored at 0-5 °C in a dark box .
Tha stability are confirmed by UV-Vis,Light scattering,TEM,SEM analitical technichs.
The SNPs synhtesised are tested and we have evidence of bacteriostatic and bactericide effect (MIC,MBC,Time killing).
Low MIC and MIB concentration (1:30;1/16) are found for any microorganism tested.
Experiment evidence an probaly mechanism of cellular damage (ultrastructure TEM analysis) are present.
