In vivo drug delivery system for colorectal cancer therapy based on doxorubicin-loaded oligonucleotide conjugated gold nanoparticles

In the present study, we have examined the effect of gold nanoparticle (AuNP)-based drug delivery system for doxorubicin (DOX) in colorectal cancer (CRC), which was prepared by using G-C rich oligonucleotide (ONT) coated... [ view full abstract ]

In the present study, we have examined the effect of gold nanoparticle (AuNP)-based drug delivery system for doxorubicin (DOX) in colorectal cancer (CRC), which was prepared by using G-C rich oligonucleotide (ONT) coated AuNPs into which DOX was loaded by intercalation (Fig. 1). ONT-modified AuNPs present numerous binding sites for DOX, thereby facilitating the delivery of significant amount of DOX to cancer cells. The anticancer effect of DOX-loaded AuNPs coated with ONTs (Doxorubicin-Oligomer-AuNP, DOA) was analyzed in human CRC cell line SW480 by using MTT assay. In vivo, inhibition ratio of tumor growth in treated-to-control (T/C) tumors was also examined in tumor-bearing mouse models.