Design and insilico modeling of Dunaliella bardawil biomass encapsulated N-succinyl chitosan nanoparticles for effective anticancer activity
Balasubramanian Somasundaram
Kalasalingam University, Krishnankoil-626126, Near Madurai,TamilNadu
Balasubramanian Somasundaram is currently a Senior Professor of Mechanical Engineering and Researcher of Sir CV Raman-KS Krishnan International Research Centre at Kalasalingam University, India. Graduated M.S and Ph.D in Metallurgical Engineering from Indian Institute of Technology-Madras, Chennai 1977 and 1983 respectively. He has a research experience as a scientist in DRDO-Naval Chemical and Materials lab, Bombay/IIT Madras from 1980 to 1984. He has 19 years of Industry and 20 years of Academic experience. Current research includes Microanalysis of bio, food materials, and nanomaterial’s of potential impact on health, and environments.
Abstract
IntroductionThe drugs with enhanced effectiveness and least side effects is one of the major tasks in current drug discovery. Microalgae have shown promising interest in food and pharmaceutical industries as well as it has... [ view full abstract ]
Introduction
The drugs with enhanced effectiveness and least side effects is one of the major tasks in current drug discovery. Microalgae have shown promising interest in food and pharmaceutical industries as well as it has shown vast potential as a sustainable energy carrier. To achieve this immense target, the N-succinyl chitosan (NSC) and bioactive compounds from Dunaliella bardawil biomass were chosen to formulate nanoparticles (NSC-NPs).
Methods
Dunaliella bardawil biomass obtained through microwave assisted extraction and the resultant bioactive compounds were analyzed using LC-MS. LC-MS chromatogram identified the individual active compounds through molecular mass and structural formula of the compounds.
Bioactive compounds encapsulated NSC-NPs were characterized to study the molecular shape, particle size, stability and polydispersity index by FTIR, XRD, SEM, TEM and Zetasize Nano analyzer. Insilico and in vitro anticancer activity studies were performed choosing 721P and liver cancer cell lines (HepG2) respectively. Further, apoptotic cell cycle analysis was carried out using Annexin V-FITC and Propidium Iodide by Flow cytometry.
Results
The obtained chromatogram revealed 6 compounds. Two major bioactive compounds β-carotene and lutein and other compounds such as carotenoids (cis and trans), phytoene, canthaxanthin, zeaxanthin and phytofluene were identified. The prepared NPs showed an average particle size of 80±5.6 nm size, spherical in shape, crystalline nature, zeta potential of -32±2.7 mV and polydispersity index of 0.51±0.02. Interestingly, the observed insilico study reports showed strong interaction of NSC-NPs and binding pockets of H-Ras P21 protooncogene. At 50 µg/mL concentration NPs displayed 95.60% cytotoxicity in HepG2 cell line. The apoptotic cell cycle analysis showed the cell death for 24hr and 48 hr representing 13.13% and 47.04% respectively.
Discussion
The highly cross-linked, biocompatible, biodegradable, nontoxic NSC-NPs were actively involved in destruction of targeted cancer cells. The obtained results showed that active NPs could enhance the controlled; site specific drug delivery and it can serve as a novel nanodrug in the management of cancer.
Authors
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Balasubramanian Somasundaram
(Kalasalingam University, Krishnankoil-626126, Near Madurai,TamilNadu)
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Selvaraj Kunjiappan
(Kalasalingam University)
Topic Areas
Targeted drug delivery and nanocarriers , Nanomedicine for cancer diagnosis & therapy , Nano-Imaging for diagnosis, therapy and delivery
Session
OS2b-207 » Nano-Imaging for diagnosis, therapy and delivery (16:30 - Tuesday, 26th September, Room 207)
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