Osteogenic differentiation of bone marrow – derived mesenchymal stem cells (BMMSCs) on nanotextured Ti6Al4V sterilized by air plasma

The Ti6Al4V surface treatment by electropolishing is able to induce a bioactive behavior using nanotexturing processes preserving the mechanical properties of Ti6Al4V. In this work, three treatments were studied to modify the... [ view full abstract ]

The Ti6Al4V surface treatment by electropolishing is able to induce a bioactive behavior using nanotexturing processes preserving the mechanical properties of Ti6Al4V. In this work, three treatments were studied to modify the Ti6Al4V surface aiming to promote the osteogenic differentiation of bone marrow – derived mesenchymal stem cells (BMMSCs) on nanotextured Ti6Al4V. Mechanical polishing and electropolishing for 4 and 12 minutes were used as surface treatments. The samples were characterized by atomic force microscopy (AFM) (Fig. 1), optical interferometry and wettability. After the air plasma sterilization process, the samples were seeded with bone marrow – derived mesenchymal stem cells (BMMSCs). The cell growth, osteogenic differentiation and mineralization (Fig. 2) were evaluated. The electropolishing surface treatments modified the nanometric morphology and wettability. The nanostructured Ti6Al4V surface, electrochemically treated for 4 minutes, presented a greater cell growth, and it also increased the osteogenesis rate and the mineralization of the BMMSCs.