Vascularized In vitro Perfusion Module (VIPM™) 3D Bio-printed with the BioAssemblyBot™

The Vascularized In-vitro Perfusion Module (VIPM™) builds upon the In-vitro Perfusion Module (IPM™) by adding a 3D printed and assembled compartment that contains isolated intact self-forming neovessels derived from living... [ view full abstract ]

The Vascularized In-vitro Perfusion Module (VIPM™) builds upon the In-vitro Perfusion Module (IPM™) by adding a 3D printed and assembled compartment that contains isolated intact self-forming neovessels derived from living microvessels and integrated with cell construct(s) of interest. The 3D bio-printing technology of BioAssemblyBot™, which is the world's first 6-axis robotic 3D bio-printer, enables a high level of engineered precision and control to create conditions for cells to behave in a prescribed manner. Further, the 3D bio-printing technology used provides a method to create a VIPM™ through completely automated means and all-in-one print run.

The vascularized and cell construct compartment is assembled at a defined location relative to the 3D printed network of channels in the IPM™. The endothelial cells lined on the IPM™ network channels are induced to sprout into the surrounding media using defined perfusion conditions. The endothelial cell sprouts will inosculate with the neovessels thereby establishing lumen continuity between the channels and the native neovasculature. Subsequently, maturation of the immature neovasculature will be driven by controlled pressure or flow across the IPM™. The resulting system is a VIPM™ that supplies blood to the cell construct(s).