Encoded SERS nanotags for multiplex bioassays

Multiplex bioassays with high throughput sequencing and high throughput screening have drawn more and more attention for the development of novel analytical techniques, which are valuable for acquisition techniques of... [ view full abstract ]

Multiplex bioassays with high throughput sequencing and high throughput screening have drawn more and more attention for the development of novel analytical techniques, which are valuable for acquisition techniques of bioinformation of precision medicine. However, detection of proteins in a wide concentration range from fg mL-1 to sub mg mL-1 is a challenge in the high throughput analysis of precision medicine. Furthermore, the ultrasensitive detection of the extremely low concentration of multiplex analytes is challenging. With the rapid development of plasmonics, surface enhanced Raman scattering (SERS) has shown its promise in biomolecular analysis as a multifunctional modality. In this talk,  SERS nanotags with fingerprinting molecular vibrational spectra as coding elements for multiplex assays will be introduced as well as their applications in practical applications with regards to the linear dynamic range (LDR), repeatability and limit of detection (LOD). Our results showed that SERS nanotags are desirable alternatives for commonly used fluorescence labels.