Maximizing library complexity down to picogram inputs with Accel-NGS 2S technology

With the ever-decreasing cost of next generation sequencing (NGS) experiments, the quantity and quality of the input DNA sample has become the limiting factor in experimental design. For NGS applications such as ChIP-Seq and... [ view full abstract ]

With the ever-decreasing cost of next generation sequencing (NGS) experiments, the quantity and quality of the input DNA sample has become the limiting factor in experimental design. For NGS applications such as ChIP-Seq and liquid biopsy, optimal input DNA quantities are as low as several picograms, making library capture efficiency crucial for maximizing library complexity while reducing introduced bias. With the Swift Biosciences Accel-NGS 2S library preparation kit, researchers are enabled to push the lower limits of DNA input into the pico scale by efficiently converting low input DNA into a highly diverse and complex library. Through our proprietary repair and ligation chemistries, Accel-NGS 2S offers unsurpassed conversion efficiency while maintaining a balanced representation of both AT-/GC-rich regions. Here, we present performance metrics of Accel-NGS 2S, along with technology comparisons, over several different sample types and inputs, revealing optimal performance.