New developments in NEBNext®: methods to enhance RNA-Seq and DNA-Seq performance

The requirements for Next Generation Sequencing library construction are continually expanding, including the need for improved performance with ever-decreasing input amounts and reduced sample quality. It is important to have... [ view full abstract ]

The requirements for Next Generation Sequencing library construction are continually expanding, including the need for improved performance with ever-decreasing input amounts and reduced sample quality. It is important to have high enzymatic efficiencies in all steps of library construction in addition to uniform coverage, regardless of GC content, input amount, or nucleic acid quality. This presentation will describe new innovations for both RNA and DNA sample preparation.  

“NEBNext Ultra II RNA” kits employ reformulated, high-efficiency reagents that are compatible with low nanogram to microgram input amounts of RNA for strand-specific library construction.

For DNA library preparation, we have developed a robust method that uses novel enzymatic fragmentation to generate the desired fragment size (regardless of input amount and with a single protocol), in combination with end repair and dA-tailing. This reliable method removes the need for costly equipment and numerous cleanup or liquid transfer steps, and reduces the time, cost and errors associated with library construction